› 5yxg

X-ray diffraction
1.48Å resolution

Crystal structure of C-terminal fragment of SpaD from Lactobacillus rhamnosus GG generated by limited proteolysis

Released:
DOI: 10.2210/pdb5yxg/pdb
Model geometry
Fit model/data
PDB entry 5yxg coloured by chain, front view.
PDB entry 5yxg coloured by chain, side view.
PDB entry 5yxg coloured by chain, top view.
Source organism: Lacticaseibacillus rhamnosus GG
Primary publication:
Bent conformation of a backbone pilin N-terminal domain supports a three-stage pilus assembly mechanism.
Chaurasia P, Pratap S, Palva A, von Ossowski I, Krishnan V
OpenAccess logo Commun Biol 1 94 (2018)
PMID: 30271975

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-219531 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pilus assembly protein Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 34.94 KDa
Source organism: Lacticaseibacillus rhamnosus GG
Expression system: Escherichia coli BL21(DE3)
Pilus assembly protein Chain: B
Molecule details ›
Chain: B
Length: 317 amino acids
Theoretical weight: 34.91 KDa
Source organism: Lacticaseibacillus rhamnosus GG
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
1 modified residue:
Ligand SME 2 x SME

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 50.106Å b: 83.16Å c: 149.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

SpaB, an atypically adhesive basal pilin from the lactobacillar SpaCBA pilus: crystallization and X-ray diffraction analysis.
Kumar Megta et al. (2019)
3 more

1 mention without citation

Bent conformation of a backbone pilin N-terminal domain supports a three-stage pilus assembly mechanism.
Chaurasia et al. (2018)