› 5z0f

X-ray diffraction
1.16Å resolution

Crystal structure of copper-bound tyrosinase from Streptomyces castaneoglobisporus in complex with the caddie protein obtained by soaking in the hydroxylamine-containing solution for 10 min at 298 K

Released:
DOI: 10.2210/pdb5z0f/pdb
Model geometry
Fit model/data
PDB entry 5z0f coloured by chain, front view.
PDB entry 5z0f coloured by chain, side view.
PDB entry 5z0f coloured by chain, top view.
Source organism: Streptomyces castaneoglobisporus
Entry authors: Matoba Y, Sugiyama M

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + 1/2 O(2) = L-dopa
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182976 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosinase copper-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 32.09 KDa
Source organism: Streptomyces castaneoglobisporus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83WS2 (Residues: 1-273; Coverage: 100%)
Sequence domains: Common central domain of tyrosinase
Structure domains: Di-copper center containing domain from catechol oxidase
MelC Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 14.11 KDa
Source organism: Streptomyces castaneoglobisporus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83WS1 (Residues: 1-126; Coverage: 100%)
Sequence domains: Tyrosinase co-factor MelC1
Structure domains: protein ne1242 domain like

Ligands and Environments

3 bound ligands:
Ligand CU 4 x CU
Ligand PER 1 x PER
Ligand NO3 8 x NO3
1 modified residue:
Ligand DAH 1 x DAH

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P21212
Unit cell:
a: 64.65Å b: 96.99Å c: 54.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.128 0.128 0.16
Expression system: Escherichia coli BL21(DE3)

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