› 5z2c

X-ray diffraction
2.59Å resolution

Crystal structure of ALPK-1 N-terminal domain in complex with ADP-heptose

Released:
DOI: 10.2210/pdb5z2c/pdb
PDB entry 5z2c coloured by chain, front view.
PDB entry 5z2c coloured by chain, side view.
PDB entry 5z2c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Alpha-kinase 1 is a cytosolic innate immune receptor for bacterial ADP-heptose.
Zhou P, She Y, Dong N, Li P, He H, Borio A, Wu Q, Lu S, Ding X, Cao Y, Xu Y, Gao W, Dong M, Ding J, Wang DC, Zamyatina A, Shao F
Nature 561 122-126 (2018)
PMID: 30111836

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-protein kinase 1 Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 446 amino acids
Theoretical weight: 49.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96QP1 (Residues: 1-446; Coverage: 36%)
Gene names: ALPK1, KIAA1527, LAK

Ligands and Environments

1 bound ligand:
Ligand AYU 9 x AYU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 169.26Å b: 214.388Å c: 173.151Å
α: 90° β: 110.33° γ: 90°
R-values:
R R work R free
0.242 0.242 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Innate immunity to intracellular LPS.
Rathinam et al. (2019)
22 more

1 mention without citation

Improving mass spectrometry analysis of protein structures with arginine-selective chemical cross-linkers.
Jones et al. (2019)