› 5z2t

X-ray diffraction
2.62Å resolution

Crystal structure of DNA-bound DUX4-HD2

Released:
DOI: 10.2210/pdb5z2t/pdb
PDB entry 5z2t coloured by chain, front view.
PDB entry 5z2t coloured by chain, side view.
PDB entry 5z2t coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of DUX4/IGH-driven transactivation.
Dong X, Zhang W, Wu H, Huang J, Zhang M, Wang P, Zhang H, Chen Z, Chen SJ, Meng G
Leukemia 32 1466-1476 (2018)
PMID: 29572508

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119476 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Double homeobox protein 4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 64 amino acids
Theoretical weight: 7.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBX2 (Residues: 94-153; Coverage: 14%)
Gene names: DUX10, DUX4
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
5'-D(*TP*TP*CP*TP*AP*AP*TP*CP*TP*AP*AP*TP*CP*TP*T)-3' Chain: E
Molecule details ›
Chain: E
Length: 15 nucleotides
Theoretical weight: 4.51 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(P*AP*AP*GP*AP*TP*TP*AP*GP*AP*TP*TP*AP*GP*T)-3' Chain: F
Molecule details ›
Chain: F
Length: 14 nucleotides
Theoretical weight: 4.34 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P41212
Unit cell:
a: 51.61Å b: 51.61Å c: 166.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.278 0.276 0.299
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Pathomechanisms and biomarkers in facioscapulohumeral muscular dystrophy: roles of DUX4 and PAX7.
Banerji et al. (2021)
3 more

4 mentions without citation

Structural basis of DUX4/IGH-driven transactivation.
Dong et al. (2018)
3 more