› 5z4z

X-ray diffraction
2.05Å resolution

Crystal structure of PaCysB NTD domain with space group C2

Released:
DOI: 10.2210/pdb5z4z/pdb
PDB entry 5z4z coloured by chain, front view.
PDB entry 5z4z coloured by chain, side view.
PDB entry 5z4z coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Molecular insights into the master regulator CysB-mediated bacterial virulence in Pseudomonas aeruginosa.
Song Y, Yang C, Chen G, Zhang Y, Seng Z, Cai Z, Zhang C, Yang L, Gan J, Liang H
Mol Microbiol 111 1195-1210 (2019)
PMID: 30618115

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator CysB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 91 amino acids
Theoretical weight: 10.31 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 106.084Å b: 53.222Å c: 57.456Å
α: 90° β: 91.66° γ: 90°
R-values:
R R work R free
0.198 0.195 0.242
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Fitting Pieces into the Puzzle of Pseudomonas aeruginosa Type III Secretion System Gene Expression.
Williams McMackin et al. (2019)
2 more