› 5z50

X-ray diffraction
2.21Å resolution

Crystal structure of PaCysB regulatory domain

Released:
DOI: 10.2210/pdb5z50/pdb
PDB entry 5z50 coloured by chain, front view.
PDB entry 5z50 coloured by chain, side view.
PDB entry 5z50 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Molecular insights into the master regulator CysB-mediated bacterial virulence in Pseudomonas aeruginosa.
Song Y, Yang C, Chen G, Zhang Y, Seng Z, Cai Z, Zhang C, Yang L, Gan J, Liang H
Mol Microbiol 111 1195-1210 (2019)
PMID: 30618115

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lysR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 26.28 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O05926 (Residues: 88-324; Coverage: 73%)
Gene names: ALP65_00768, CAZ10_29930, DT376_27155, GNQ48_00780, GUL26_20460, IPC1295_05530, IPC737_29080, NCTC13621_01794, PAERUG_P19_London_7_VIM_2_05_10_03160, cysB
Sequence domains: LysR substrate binding domain
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand SO4 9 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P43212
Unit cell:
a: 65.718Å b: 65.718Å c: 259.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Fitting Pieces into the Puzzle of Pseudomonas aeruginosa Type III Secretion System Gene Expression.
Williams McMackin et al. (2019)
2 more

1 mention without citation

Exploring the genetic determinants underlying the differential production of an inducible chromosomal cephalosporinase - BlaB in Yersinia enterocolitica biotypes 1A, 1B, 2 and 4.
Singhal et al. (2020)