› 5z6h

X-ray diffraction
2.3Å resolution

Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form

Released:
DOI: 10.2210/pdb5z6h/pdb
PDB entry 5z6h coloured by chain, front view.
PDB entry 5z6h coloured by chain, side view.
PDB entry 5z6h coloured by chain, top view.
Source organism: Enterobacter cloacae
Primary publication:
Mechanistic insights into enzymatic catalysis by trehalase from the insect gut endosymbiont Enterobacter cloacae.
Adhav A, Harne S, Bhide A, Giri A, Gayathri P, Joshi R
FEBS J 286 1700-1716 (2019)
PMID: 30657252

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic trehalase Chains: A, B
Molecule details ›
Chains: A, B
Length: 563 amino acids
Theoretical weight: 63.17 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
Structure domains: Glycosyltransferase

Ligands and Environments

2 bound ligands:
Ligand SO4 10 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 55.25Å b: 127.909Å c: 156.139Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.168 0.229
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Bacterial α-diglucoside metabolism: perspectives and potential for biotechnology and biomedicine.
Garcia et al. (2021)
6 other articles

1 mention without citation

Plant drought tolerance provided through genome editing of the trehalase gene.
Nuñez-Muñoz et al. (2021)