› 5z6q

X-ray diffraction
3Å resolution

Crystal structure of AAA of Spastin

Released:
DOI: 10.2210/pdb5z6q/pdb
PDB entry 5z6q coloured by chain, front view.
PDB entry 5z6q coloured by chain, side view.
PDB entry 5z6q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The AAA protein spastin possesses two levels of basal ATPase activity.
Fan X, Lin Z, Fan G, Lu J, Hou Y, Habai G, Sun L, Yu P, Shen Y, Wen M, Wang C
FEBS Lett 592 1625-1633 (2018)
PMID: 29710391

Function and Biology Details

Reaction catalysed: 
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spastin Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 42.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9UBP0 (Residues: 229-616; Coverage: 63%)
Gene names: ADPSP, FSP2, KIAA1083, SPAST, SPG4
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P65
Unit cell:
a: 90.52Å b: 90.52Å c: 89.153Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.194 0.276
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

1 review citation

Hereditary Spastic Paraplegia: An Update.
Meyyazhagan et al. (2022)

1 mention without citation

Structure of spastin bound to a glutamate-rich peptide implies a hand-over-hand mechanism of substrate translocation.
Han et al. (2020)