› 5z7w

X-ray diffraction
1.66Å resolution

Crystal structure of Striga hermonthica HTL1 (ShHTL1)

Released:
DOI: 10.2210/pdb5z7w/pdb
PDB entry 5z7w coloured by chain, front view.
PDB entry 5z7w coloured by chain, side view.
PDB entry 5z7w coloured by chain, top view.
Source organism: Striga hermonthica
Primary publication:
Structural analysis of HTL and D14 proteins reveals the basis for ligand selectivity in Striga.
Xu Y, Miyakawa T, Nosaki S, Nakamura A, Lyu Y, Nakamura H, Ohto U, Ishida H, Shimizu T, Asami T, Tanokura M
Nat Commun 9 3947 (2018)
PMID: 30258184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 277 amino acids
Theoretical weight: 30.4 KDa
Source organism: Striga hermonthica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0M4AV81 (Residues: 1-272; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand NA 3 x NA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 81.17Å b: 138.77Å c: 49.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The Many Models of Strigolactone Signaling.
Bürger et al. (2020)
7 more

2 mentions without citation

In-silico analysis of the strigolactone ligand-receptor system.
Bürger et al. (2020)
1 more