› 5z99

X-ray diffraction
1.49Å resolution

Crystal structure of Sialic acid Binding protein from Haemophilus ducreyi with Neu5Ac

Released:
DOI: 10.2210/pdb5z99/pdb
PDB entry 5z99 coloured by chain, front view.
PDB entry 5z99 coloured by chain, side view.
PDB entry 5z99 coloured by chain, top view.
Source organism: [Haemophilus] ducreyi 35000HP
Primary publication:
Molecular characterization of the interaction of sialic acid with the periplasmic binding protein from Haemophilus ducreyi.
Gangi Setty T, Mowers JC, Hobbs AG, Maiya SP, Syed S, Munson RS, Apicella MA, Subramanian R
J Biol Chem 293 20073-20084 (2018)
PMID: 30315109

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182065 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute-binding protein family 5 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 485 amino acids
Theoretical weight: 54.07 KDa
Source organism: [Haemophilus] ducreyi 35000HP
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7VL18 (Residues: 41-525; Coverage: 92%)
Gene name: HD_1669
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle

Ligands and Environments

1 bound ligand:
Ligand SLB 1 x SLB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 73.368Å b: 89.63Å c: 90.787Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.194
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Bacterial Sialic Acid Catabolism at the Host-Microbe Interface.
Kim et al. (2023)
3 other articles

3 mentions without citation

Biochemical and structural basis of sialic acid utilization by gut microbes.
Bell et al. (2023)
2 more