› 5z9h

X-ray diffraction
1.49Å resolution

Crystal structure of KAI2_ply2(A219V)

Released:
DOI: 10.2210/pdb5z9h/pdb
PDB entry 5z9h coloured by chain, front view.
PDB entry 5z9h coloured by chain, side view.
PDB entry 5z9h coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
A missense allele of KARRIKIN-INSENSITIVE2 impairs ligand-binding and downstream signaling in Arabidopsis thaliana.
Lee I, Kim K, Lee S, Lee S, Hwang E, Shin K, Kim D, Choi J, Choi H, Cha JS, Kim H, Lee RA, Jeong S, Kim J, Kim Y, Nam HG, Park SK, Cho HS, Soh MS
J Exp Bot 69 3609-3623 (2018)
PMID: 29722815

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable esterase KAI2 Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 29.84 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SZU7 (Residues: 1-270; Coverage: 100%)
Gene names: At4g37470, D14L, F6G17.120, HTL, KAI2
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 50.842Å b: 55.494Å c: 53.019Å
α: 90° β: 116.42° γ: 90°
R-values:
R R work R free
0.136 0.134 0.168
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The mechanism of host-induced germination in root parasitic plants.
Nelson DC. (2021)
2 more

2 mentions without citation

A missense allele of KARRIKIN-INSENSITIVE2 impairs ligand-binding and downstream signaling in Arabidopsis thaliana.
Lee et al. (2018)
1 more