› 5z9j

X-ray diffraction
2Å resolution

Identification of the functions of unusual cytochrome p450-like monooxygenases involved in microbial secondary metablism

Released:
DOI: 10.2210/pdb5z9j/pdb
PDB entry 5z9j coloured by chain, front view.
PDB entry 5z9j coloured by chain, side view.
PDB entry 5z9j coloured by chain, top view.
Source organism: Streptomyces himastatinicus ATCC 53653
Primary publication:
Riboflavin Is Directly Involved in N-Dealkylation Catalyzed by Bacterial Cytochrome P450 Monooxygenases.
Zhang C, Lu M, Lin L, Huang Z, Zhang R, Wu X, Chen Y
Chembiochem 21 2297-2305 (2020)
PMID: 32243060

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123857 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative P450-like enzyme Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 398 amino acids
Theoretical weight: 44.74 KDa
Source organism: Streptomyces himastatinicus ATCC 53653
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0LWB2 (Residues: 2-397; Coverage: 100%)
Gene name: hmtS
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand TAM 2 x TAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P1
Unit cell:
a: 56.956Å b: 78.469Å c: 97.68Å
α: 78.27° β: 72.63° γ: 69.46°
R-values:
R R work R free
0.223 0.222 0.262
Expression system: Escherichia coli BL21(DE3)

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