› 5zbs

X-ray diffraction
2.2Å resolution

Crystal Structure of Kinesin-3 KIF13B motor Y73C mutant

Released:
DOI: 10.2210/pdb5zbs/pdb
PDB entry 5zbs coloured by chain, front view.
PDB entry 5zbs coloured by chain, side view.
PDB entry 5zbs coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural Delineation of the Neck Linker of Kinesin-3 for Processive Movement.
Ren J, Zhang Y, Wang S, Huo L, Lou J, Feng W
J Mol Biol 430 2030-2041 (2018)
PMID: 29752968

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin family member 13B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 375 amino acids
Theoretical weight: 41.52 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0G2K8Z9 (Residues: 12-41, 42-358; Coverage: 19%)
Gene name: Kif13b
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

2 bound ligands:
Ligand MG 5 x MG
Ligand ANP 3 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P32
Unit cell:
a: 107.311Å b: 107.311Å c: 91.599Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.172 0.22
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Cellular functions of actin- and microtubule-associated septins.
Spiliotis et al. (2021)
1 more