› 5zbz

X-ray diffraction
1.31Å resolution

Crystal structure of the DEAD domain of Human eIF4A with sanguinarine

Released:
DOI: 10.2210/pdb5zbz/pdb
PDB entry 5zbz coloured by chain, front view.
PDB entry 5zbz coloured by chain, side view.
PDB entry 5zbz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Targeting the N Terminus of eIF4AI for Inhibition of Its Catalytic Recycling.
Jiang C, Tang Y, Ding L, Tan R, Li X, Lu J, Jiang J, Cui Z, Tang Z, Li W, Cao Z, Schneider-Poetsch T, Jiang W, Luo C, Ding Y, Liu J, Dang Y
Cell Chem Biol 26 1417-1426.e5 (2019)
PMID: 31402318

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158100 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic initiation factor 4A-I Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 24.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60842 (Residues: 20-238; Coverage: 54%)
Gene names: DDX2A, EIF4A, EIF4A1
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand SAU 1 x SAU
Ligand MLI 1 x MLI
1 modified residue:
Ligand MLY 14 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2
Unit cell:
a: 73.471Å b: 56.63Å c: 66.324Å
α: 90° β: 116.775° γ: 90°
R-values:
R R work R free
0.194 0.194 0.21
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Targeting the DEAD-Box RNA Helicase eIF4A with Rocaglates-A Pan-Antiviral Strategy for Minimizing the Impact of Future RNA Virus Pandemics.
Taroncher-Oldenburg et al. (2021)
1 more