› 5ze4

X-ray diffraction
2.11Å resolution

The structure of holo- structure of DHAD complex with [2Fe-2S] cluster

Released:
DOI: 10.2210/pdb5ze4/pdb
PDB entry 5ze4 coloured by chain, front view.
PDB entry 5ze4 coloured by chain, side view.
PDB entry 5ze4 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Resistance-gene-directed discovery of a natural-product herbicide with a new mode of action.
Yan Y, Liu Q, Zang X, Yuan S, Bat-Erdene U, Nguyen C, Gan J, Zhou J, Jacobsen SE, Tang Y
Nature 559 415-418 (2018)
PMID: 29995859

Function and Biology Details

Reaction catalysed: 
2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192279 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroxy-acid dehydratase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 573 amino acids
Theoretical weight: 61.01 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q9LIR4 (Residues: 36-608; Coverage: 94%)
Gene names: At3g23940, DHAD, F14O13.13
Sequence domains: Dehydratase family

Ligands and Environments

4 bound ligands:
Ligand FES 1 x FES
Ligand SO4 3 x SO4
Ligand ACT 1 x ACT
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P42212
Unit cell:
a: 135.467Å b: 135.467Å c: 65.978Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.215
Expression system: Escherichia coli K-12

Quick links

Citations

23 review citations

Mining and unearthing hidden biosynthetic potential.
Scherlach et al. (2021)
22 more

5 mentions without citation

Review on Structures of Pesticide Targets.
Li et al. (2020)
4 more