› 5zfz

X-ray diffraction
1.9Å resolution

Crystal structure of human DUX4 homeodomains bound to A12T DNA mutant

Released:
DOI: 10.2210/pdb5zfz/pdb
PDB entry 5zfz coloured by chain, front view.
PDB entry 5zfz coloured by chain, side view.
PDB entry 5zfz coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for multiple gene regulation by human DUX4.
Li Y, Wu B, Liu H, Gao Y, Yang C, Chen X, Zhang J, Chen Y, Gu Y, Li J, Ma J, Gan J
Biochem Biophys Res Commun 505 1161-1167 (2018)
PMID: 30322619

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119506 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Double homeobox protein 4 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 17.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBX2 (Residues: 1-149; Coverage: 35%)
Gene names: DUX10, DUX4
Sequence domains: Homeodomain
DNA (5'-D(*CP*CP*AP*CP*TP*AP*AP*CP*CP*TP*AP*TP*TP*CP*AP*CP*AP*CP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.65 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*GP*TP*GP*TP*GP*AP*AP*TP*AP*GP*GP*TP*TP*AP*GP*TP*GP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 19 nucleotides
Theoretical weight: 6 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P3221
Unit cell:
a: 65.013Å b: 65.013Å c: 151.661Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.221
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

DUX4 Role in Normal Physiology and in FSHD Muscular Dystrophy.
Mocciaro et al. (2021)
2 more