› 5zg8

X-ray diffraction
2.4Å resolution

Crystal Structure of TtNRS

Released:
DOI: 10.2210/pdb5zg8/pdb
PDB entry 5zg8 coloured by chain, front view.
PDB entry 5zg8 coloured by chain, side view.
PDB entry 5zg8 coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Mutharasappan N, Jain V, Sharma A, Manickam Y, Jeyaraman J

Function and Biology Details

Reaction catalysed: 
ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156968 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Asparagine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 438 amino acids
Theoretical weight: 50.86 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54263 (Residues: 1-438; Coverage: 100%)
Gene names: TTHA0708, asnS
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P3121
Unit cell:
a: 67.294Å b: 67.294Å c: 189.117Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.155 0.209
Expression system: Escherichia coli BL21(DE3)

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