› 5zht

X-ray diffraction
1.53Å resolution

Crystal structure of OsD14 in complex with covalently bound KK073

Released:
DOI: 10.2210/pdb5zht/pdb
PDB entry 5zht coloured by chain, front view.
PDB entry 5zht coloured by chain, side view.
PDB entry 5zht coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Triazole Ureas Covalently Bind to Strigolactone Receptor and Antagonize Strigolactone Responses.
Nakamura H, Hirabayashi K, Miyakawa T, Kikuzato K, Hu W, Xu Y, Jiang K, Takahashi I, Niiyama R, Dohmae N, Tanokura M, Asami T
Mol Plant 12 44-58 (2019)
PMID: 30391752

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strigolactone esterase D14 Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.02 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q10QA5 (Residues: 54-318; Coverage: 83%)
Gene names: D14, D88, HTD2, LOC_Os03g10620, Os03g0203200
Sequence domains: Alpha/beta hydrolase family

Ligands and Environments

1 bound ligand:
Ligand 9GU 1 x 9GU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P3221
Unit cell:
a: 49.349Å b: 49.349Å c: 192.469Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.195 0.221
Expression system: Escherichia coli

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Citations

3 review citations

The mechanism of host-induced germination in root parasitic plants.
Nelson DC. (2021)
2 more