› 5zi1

X-ray diffraction
2.3Å resolution

Crystal structure of Bacillus thuringiensis insecticidal crystal protein Cry7Ca1 (wild type)

Released:
DOI: 10.2210/pdb5zi1/pdb
PDB entry 5zi1 coloured by chain, front view.
PDB entry 5zi1 coloured by chain, side view.
PDB entry 5zi1 coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
Crystal structure of Bacillus thuringiensis Cry7Ca1 toxin active against Locusta migratoria manilensis.
Jing X, Yuan Y, Wu Y, Wu D, Gong P, Gao M
Protein Sci 28 609-619 (2019)
PMID: 30506755

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-109032 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Crystaline entomocidal protoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 609 amino acids
Theoretical weight: 68.8 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli M15
UniProt:
  • Canonical: A9Q1Y3 (Residues: 55-641; Coverage: 51%)
Gene name: cry
Sequence domains:
Structure domains: Pesticidal crystal protein, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: C2
Unit cell:
a: 126.261Å b: 235.959Å c: 69.025Å
α: 90° β: 116.53° γ: 90°
R-values:
R R work R free
0.191 0.189 0.233
Expression system: Escherichia coli M15

Quick links

Citations

3 review citations

Making 3D-Cry Toxin Mutants: Much More Than a Tool of Understanding Toxins Mechanism of Action.
Vílchez S. (2020)
2 more

1 mention without citation

Crystal structure of Bacillus thuringiensis Cry7Ca1 toxin active against Locusta migratoria manilensis.
Jing et al. (2019)