› 5zjk

X-ray diffraction
2.6Å resolution

Structure of myroilysin

Released:
DOI: 10.2210/pdb5zjk/pdb
PDB entry 5zjk coloured by chain, front view.
PDB entry 5zjk coloured by chain, side view.
PDB entry 5zjk coloured by chain, top view.
Source organism: Myroides sp. CSLB8
Primary publication:
Crystal structure of mature myroilysin and implication for its activation mechanism.
Ran T, Li W, Sun B, Xu M, Qiu S, Xu DQ, He J, Wang W
Int J Biol Macromol 156 1556-1564 (2020)
PMID: 31785296

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase metallopeptidase domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R
Length: 213 amino acids
Theoretical weight: 24.08 KDa
Source organism: Myroides sp. CSLB8
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0P0DZ84 (Residues: 69-273; Coverage: 75%)
Sequence domains: Astacin (Peptidase family M12A)

Ligands and Environments

2 bound ligands:
Ligand ZN 18 x ZN
Ligand PO4 12 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 125.176Å b: 115.902Å c: 163.354Å
α: 90° β: 93.71° γ: 90°
R-values:
R R work R free
0.19 0.187 0.254
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structural and evolutionary insights into astacin metallopeptidases.
Gomis-Rüth et al. (2022)

1 mention without citation

Zymogenic latency in an ∼250-million-year-old astacin metallopeptidase.
Guevara et al. (2022)