› 5zlp

X-ray diffraction
2.93Å resolution

Crystal structure of glutamine synthetase from helicobacter pylori

Released:
DOI: 10.2210/pdb5zlp/pdb
PDB entry 5zlp coloured by chain, front view.
PDB entry 5zlp coloured by chain, side view.
PDB entry 5zlp coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Structural Analysis of Glutamine Synthetase from Helicobacter pylori.
Joo HK, Park YW, Jang YY, Lee JY
Sci Rep 8 11657 (2018)
PMID: 30076387

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine synthetase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 481 amino acids
Theoretical weight: 54.58 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: P94845 (Residues: 1-481; Coverage: 100%)
Gene names: HP_0512, glnA
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ATP 6 x ATP
Ligand ADP 6 x ADP
Ligand PPQ 3 x PPQ
Ligand MG 4 x MG
Ligand P3P 1 x P3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: C2
Unit cell:
a: 234.409Å b: 135.167Å c: 203.08Å
α: 90° β: 91.61° γ: 90°
R-values:
R R work R free
0.172 0.168 0.246
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Polyamine and Ethanolamine Metabolism in Bacteria as an Important Component of Nitrogen Assimilation for Survival and Pathogenicity.
Krysenko et al. (2022)
8 other articles

2 mentions without citation

Structural Analysis of Glutamine Synthetase from Helicobacter pylori.
Joo et al. (2018)
1 more