› 5zoa

X-ray diffraction
1.54Å resolution

The crystal structure of a Thermobifida fusca cutinase

Released:
DOI: 10.2210/pdb5zoa/pdb
PDB entry 5zoa coloured by chain, front view.
PDB entry 5zoa coloured by chain, side view.
PDB entry 5zoa coloured by chain, top view.
Source organism: Thermobifida fusca
Entry authors: Dong QL, Wu L, Wu J, Zhou JH

Function and Biology Details

Reactions catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179387 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase cut2 Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.2 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q6A0I4 (Residues: 41-301; Coverage: 100%)
Gene names: TfH, bta1, cut1, cut2
Sequence domains: Chlorophyllase
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P422
Unit cell:
a: 86.298Å b: 86.298Å c: 76.117Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.166 0.18
Expression system: Escherichia coli K-12

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