› 5zrc

X-ray diffraction
1.1Å resolution

Structural insights into the catalysis mechanism of M. smegmatis antimutator protein MutT2

Released:
DOI: 10.2210/pdb5zrc/pdb
PDB entry 5zrc coloured by chain, front view.
PDB entry 5zrc coloured by chain, side view.
PDB entry 5zrc coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structural insights into the specificity and catalytic mechanism of mycobacterial nucleotide pool sanitizing enzyme MutT2.
Singh A, Mohammad Arif S, Biak Sang P, Varshney U, Vijayan M
J Struct Biol 204 449-456 (2018)
PMID: 30312643

Function and Biology Details

Reaction catalysed: 
8-oxo-dGTP + H(2)O = 8-oxo-dGMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
8-oxo-dGTP diphosphatase Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.08 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0R2K6 (Residues: 1-130; Coverage: 100%)
Gene name: MSMEG_5148
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21212
Unit cell:
a: 65.84Å b: 59.87Å c: 31.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.142 0.172
Expression system: Escherichia coli BL21

Quick links

Citations

1 mention without citation

Preparation, Mechanical Properties and Strengthening Mechanism of W-Re Alloys: A Review.
Zheng et al. (2023)