› 5zrs

X-ray diffraction
1.4Å resolution

Crystal structure of PET-degrading cutinase Cut190 S176A/S226P/R228S mutant in monoethyl adipate bound state

Released:
DOI: 10.2210/pdb5zrs/pdb
PDB entry 5zrs coloured by chain, front view.
PDB entry 5zrs coloured by chain, side view.
PDB entry 5zrs coloured by chain, top view.
Source organism: Saccharomonospora viridis
Primary publication:
Structural Dynamics of the PET-Degrading Cutinase-like Enzyme from Saccharomonospora viridis AHK190 in Substrate-Bound States Elucidates the Ca2+-Driven Catalytic Cycle.
Numoto N, Kamiya N, Bekker GJ, Yamagami Y, Inaba S, Ishii K, Uchiyama S, Kawai F, Ito N, Oda M
Biochemistry 57 5289-5300 (2018)
PMID: 30110540

Function and Biology Details

Reaction catalysed: 
Cutin + H(2)O = cutin monomers
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cutinase Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.21 KDa
Source organism: Saccharomonospora viridis
Expression system: Escherichia coli
UniProt:
  • Canonical: W0TJ64 (Residues: 45-304; Coverage: 86%)
Gene names: Cut190, MINT15_00360
Sequence domains: Platelet-activating factor acetylhydrolase, isoform II
Structure domains: alpha/beta hydrolase

Ligands and Environments

4 bound ligands:
Ligand 9J6 1 x 9J6
Ligand ZN 3 x ZN
Ligand CA 1 x CA
Ligand GOL 2 x GOL
1 modified residue:
Ligand MHO 1 x MHO

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 50.457Å b: 63.856Å c: 78.785Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.192
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Mechanism-Based Design of Efficient PET Hydrolases.
Wei et al. (2022)
2 more

5 mentions without citation

Current knowledge on enzymatic PET degradation and its possible application to waste stream management and other fields.
Kawai et al. (2019)
4 more