› 5zsz

X-ray diffraction
2.4Å resolution

Catechol 2,3-dioxygenase (C23O64) from Diaphorobacter sp DS2

Released:
DOI: 10.2210/pdb5zsz/pdb
PDB entry 5zsz coloured by chain, front view.
PDB entry 5zsz coloured by chain, side view.
PDB entry 5zsz coloured by chain, top view.
Source organism: Diaphorobacter sp. DS2
Entry authors: Mishra K, Arya CK, Subramanian R, Ramanathan G

Function and Biology Details

Reaction catalysed: 
Catechol + O(2) = 2-hydroxymuconate-6-semialdehyde
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105279 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metapyrocatechase Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 32.55 KDa
Source organism: Diaphorobacter sp. DS2
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A509GUM5 (Residues: 1-293; Coverage: 100%)
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P6222
Unit cell:
a: 102.454Å b: 102.454Å c: 113.348Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.2 0.241
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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