› 5ztm

X-ray diffraction
2.9Å resolution

Crystal structure of MLE dsRBDs in complex with roX2 (R2H1)

Released:
DOI: 10.2210/pdb5ztm/pdb
PDB entry 5ztm coloured by chain, front view.
PDB entry 5ztm coloured by chain, side view.
PDB entry 5ztm coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural insights reveal the specific recognition of roX RNA by the dsRNA-binding domains of the RNA helicase MLE and its indispensable role in dosage compensation in Drosophila.
Lv M, Yao Y, Li F, Xu L, Yang L, Gong Q, Xu YZ, Shi Y, Fan YJ, Tang Y
Nucleic Acids Res 47 3142-3157 (2019)
PMID: 30649456

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150291 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Dosage compensation regulator mle Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.32 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P24785 (Residues: 1-264; Coverage: 20%)
Gene names: CG11680, mle, nap
Sequence domains: Double-stranded RNA binding motif
non-coding mRNA sequence roX2 Chain: C
Molecule details ›
Chain: C
Length: 55 nucleotides
Theoretical weight: 17.72 KDa
Source organism: Drosophila melanogaster

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: R32
Unit cell:
a: 108.479Å b: 108.479Å c: 347.061Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.191 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

When Down Is Up: Heterochromatin, Nuclear Organization and X Upregulation.
Makki et al. (2021)
1 more

2 mentions without citation

Structural insights reveal the specific recognition of roX RNA by the dsRNA-binding domains of the RNA helicase MLE and its indispensable role in dosage compensation in Drosophila.
Lv et al. (2019)
1 more