› 5zty

X-ray diffraction
2.8Å resolution

Crystal structure of human G protein coupled receptor

Released:
DOI: 10.2210/pdb5zty/pdb
PDB entry 5zty coloured by chain, front view.
PDB entry 5zty coloured by chain, side view.
PDB entry 5zty coloured by chain, top view.
Source organisms:
Primary publication:
Crystal Structure of the Human Cannabinoid Receptor CB2.
Li X, Hua T, Vemuri K, Ho JH, Wu Y, Wu L, Popov P, Benchama O, Zvonok N, Locke K, Qu L, Han GW, Iyer MR, Cinar R, Coffey NJ, Wang J, Wu M, Katritch V, Zhao S, Kunos G, Bohn LM, Makriyannis A, Stevens RC, Liu ZJ
Cell 176 459-467.e13 (2019)
PMID: 30639103

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112942 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cannabinoid receptor 2; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 56.2 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P34972 (Residues: 21-222, 235-325; Coverage: 81%)
  • Canonical: D9IEF7 (Residues: 2-161; Coverage: 98%)
Gene names: CB2A, CB2B, CNR2, T4Tp126, e
Sequence domains:

Ligands and Environments

7 bound ligands:
Ligand 9JU 1 x 9JU
Ligand OLA 1 x OLA
Ligand OLC 3 x OLC
Ligand PEG 2 x PEG
Ligand EPE 2 x EPE
Ligand PG4 1 x PG4
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 34.295Å b: 106.24Å c: 183.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.275
Expression system: Spodoptera frugiperda

Quick links

Citations

49 review citations

The therapeutic potential of second and third generation CB1R antagonists.
Cinar et al. (2020)
48 more

48 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
47 more