› 5zzk

X-ray diffraction
2.64Å resolution

A Con Artist: Phenylphenoxybenzamide is not a Glycosyltransferase Inhibitor

Released:
DOI: 10.2210/pdb5zzk/pdb
Model geometry
Fit model/data
PDB entry 5zzk coloured by chain, front view.
PDB entry 5zzk coloured by chain, side view.
PDB entry 5zzk coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
A Con Artist: Phenylphenoxybenzamide is not a Glycosyltransferase Inhibitor
Wybenga GG, Wu WS
Biorxiv (2018)

Function and Biology Details

Reaction catalysed: 
[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans,octa-cis-undecaprenyl diphosphate +di-trans,octa-cis-undecaprenyl diphosphate + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monofunctional glycosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.43 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99T05 (Residues: 28-269; Coverage: 90%)
Gene names: SAV1874, mgt
Sequence domains: Transglycosylase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P21212
Unit cell:
a: 153.88Å b: 39.64Å c: 55.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.245 0.327
Expression system: Escherichia coli

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