› 6a0w

X-ray diffraction
2Å resolution

Crystal structure of lipase from Rhizopus microsporus var. chinensis

Released:
DOI: 10.2210/pdb6a0w/pdb
PDB entry 6a0w coloured by chain, front view.
PDB entry 6a0w coloured by chain, side view.
PDB entry 6a0w coloured by chain, top view.
Source organism: Rhizopus microsporus var. chinensis
Primary publication:
Structural Basis by Which the N-Terminal Polypeptide Segment of Rhizopus chinensis Lipase Regulates Its Substrate Binding Affinity.
Zhang M, Yu XW, Xu Y, Guo RT, Swapna GVT, Szyperski T, Hunt JF, Montelione GT
Biochemistry 58 3943-3954 (2019)
PMID: 31436959

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fungal lipase-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 33.36 KDa
Source organism: Rhizopus microsporus var. chinensis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A3FM73 (Residues: 94-389; Coverage: 82%)
Sequence domains: Lipase (class 3)

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P3121
Unit cell:
a: 86.225Å b: 86.225Å c: 101.151Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.168 0.208
Expression system: Komagataella pastoris

Quick links

Citations

2 citation in other articles

Effectors of the Stenotrophomonas maltophilia Type IV Secretion System Mediate Killing of Clinical Isolates of Pseudomonas aeruginosa.
Nas et al. (2021)
1 more

7 mentions without citation

Structural Basis by Which the N-Terminal Polypeptide Segment of Rhizopus chinensis Lipase Regulates Its Substrate Binding Affinity.
Zhang et al. (2019)
6 more