› 6a6h

X-ray diffraction
2.31Å resolution

Crystal Structure of Swine Major Histocompatibility Complex Class I SLA-2*040202 For 2.3 Angstrom

Released:
DOI: 10.2210/pdb6a6h/pdb
PDB entry 6a6h coloured by chain, front view.
PDB entry 6a6h coloured by chain, side view.
PDB entry 6a6h coloured by chain, top view.
Source organisms:
Primary publication:
Crystallization of SLA-2*04:02:02 complexed with a CTL epitope derived from FMDV.
Ning S, Wang ZB, Qi P, Xiao J, Wang XJ
Res Vet Sci 128 90-98 (2020)
PMID: 31760318

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-155916 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.55 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8MHU4 (Residues: 25-299; Coverage: 81%)
Gene names: SLA-2, SLA-B
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 98 amino acids
Theoretical weight: 11.43 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07717 (Residues: 21-118; Coverage: 100%)
Gene name: B2M
Sequence domains: Immunoglobulin C1-set domain
Capsid protein VP2 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.03 KDa
Source organism: Foot-and-mouth disease virus A
Expression system: Not provided
UniProt:
  • Canonical: P49303 (Residues: 440-448; Coverage: 0%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3221
Unit cell:
a: 101.802Å b: 101.802Å c: 73.455Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.199 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Peptide-Based Vaccines: Foot-and-Mouth Disease Virus, a Paradigm in Animal Health.
Forner et al. (2021)
1 more

1 mention without citation

Porcine Parvovirus 7: Evolutionary Dynamics and Identification of Epitopes toward Vaccine Design.
Wang et al. (2020)