› 6a8x

X-ray diffraction
2.35Å resolution

Crystal structure of a apo form cyclase from Fischerella sp.

Released:
DOI: 10.2210/pdb6a8x/pdb
PDB entry 6a8x coloured by chain, front view.
PDB entry 6a8x coloured by chain, side view.
PDB entry 6a8x coloured by chain, top view.
Source organism: Fischerella ambigua
Primary publication:
The Crystal Structure of a Class of Cyclases that Catalyze the Cope Rearrangement.
Chen CC, Hu X, Tang X, Yang Y, Ko TP, Gao J, Zheng Y, Huang JW, Yu Z, Li L, Han S, Cai N, Zhang Y, Liu W, Guo RT
Angew Chem Int Ed Engl 57 15060-15064 (2018)
PMID: 30222239

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103543 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FidU1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 24.24 KDa
Source organism: Fischerella ambigua
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1L1VVY8 (Residues: 27-228; Coverage: 89%)
Gene name: fimC5

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P41212
Unit cell:
a: 90.993Å b: 90.993Å c: 234.496Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Terpene synthases in disguise: enzymology, structure, and opportunities of non-canonical terpene synthases.
Rudolf et al. (2020)
1 more