6a96

Electron Microscopy
3.51Å resolution

Cryo-EM structure of the human alpha5beta3 GABAA receptor in complex with GABA and Nb25

Released:
Source organisms:
Primary publication:
Cryo-EM structure of the human α5β3 GABAA receptor.
Cell Res 28 958-961 (2018)
PMID: 30140029
Related structures: EMD-6998

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-210964 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (7 distinct):
Nb25 Chains: K, L, O
Molecule details ›
Chains: K, L, O
Length: 125 amino acids
Theoretical weight: 13.67 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Gamma-aminobutyric acid receptor subunit beta-3 Chains: B, C, D, E
Molecule details ›
Chains: B, C, D, E
Length: 366 amino acids
Theoretical weight: 42.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P28472 (Residues: 1-332, 447-473; Coverage: 75%)
Gene name: GABRB3
Sequence domains:
Gamma-aminobutyric acid receptor subunit alpha-5 Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 44.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P31644 (Residues: 1-346, 424-462; Coverage: 82%)
Gene name: GABRA5
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.51Å
Relevant EMDB volumes: EMD-6998
Expression systems:
  • Escherichia coli
  • Homo sapiens