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› 6abv

X-ray diffraction
1.8Å resolution

Crystal structure of citrate synthase (Msed_0281) from Metallosphaera sedula

Released:
DOI: 10.2210/pdb6abv/pdb
Model geometry
Fit model/data
PDB entry 6abv coloured by chain, front view.
PDB entry 6abv coloured by chain, side view.
PDB entry 6abv coloured by chain, top view.
Source organism: Metallosphaera sedula DSM 5348
Primary publication:
Crystal structure and biochemical properties of msed_0281, the citrate synthase from Metallosphaera sedula.
Lee SH, Kim KJ
Biochem Biophys Res Commun 509 722-727 (2019)
PMID: 30611567

Function and Biology Details

Reaction catalysed: 
oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate synthase Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 43.63 KDa
Source organism: Metallosphaera sedula DSM 5348
Expression system: Escherichia coli
UniProt:
  • Canonical: A4YDF6 (Residues: 2-378; Coverage: 100%)
Gene name: Msed_0281
Sequence domains: Citrate synthase, C-terminal domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2221
Unit cell:
a: 61.711Å b: 93.622Å c: 130.228Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.223
Expression system: Escherichia coli

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Citations

1 review citation

Creating de novo peptide-based bioactivities: from assembly to origami.
Ma et al. (2022)
1 other article