6ac8

X-ray diffraction
2.75Å resolution

Crystal structure of Mycobacterium smegmatis Mfd at 2.75 A resolution

Released:
Entry authors: Putta S, Fox GC, Walsh MA, Rao DN, Nagaraja V, Natesh R

Function and Biology Details

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-106439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription-repair-coupling factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 1235 amino acids
Theoretical weight: 133.44 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0R3C5 (Residues: 1-1215; Coverage: 100%)
Gene names: MSMEG_5423, mfd
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 83.855Å b: 162.026Å c: 215.397Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.259
Expression system: Escherichia coli