6aeb

X-ray diffraction
3Å resolution

Crystal structure of xCas9 in complex with sgRNA and target DNA (AAG PAM)

Released:
Source organism: Streptococcus pyogenes
Primary publication:
Structural insights into a high fidelity variant of SpCas9.
Cell Res 29 183-192 (2019)
PMID: 30664728

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-119553 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chains: B, F
Molecule details ›
Chains: B, F
Length: 1368 amino acids
Theoretical weight: 158.54 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
DNA (25-MER) Chains: C, G
Molecule details ›
Chains: C, G
Length: 28 nucleotides
Theoretical weight: 8.56 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
DNA (5'-D(*AP*AP*AP*AP*AP*GP*TP*AP*TP*TP*G)-3') Chains: D, H
Molecule details ›
Chains: D, H
Length: 11 nucleotides
Theoretical weight: 3.41 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
RNA (95-MER) Chains: A, E
Molecule details ›
Chains: A, E
Length: 100 nucleotides
Theoretical weight: 32.27 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 361.853Å b: 71.13Å c: 198.32Å
α: 90° β: 101.75° γ: 90°
R-values:
R R work R free
0.232 0.231 0.283
Expression systems:
  • Escherichia coli
  • Not provided