› 6aee

X-ray diffraction
3.3Å resolution

Crystal structure of the four Ig-like domains of LILRB1 complexed with HLA-G

Released:
DOI: 10.2210/pdb6aee/pdb
PDB entry 6aee coloured by chain, front view.
PDB entry 6aee coloured by chain, side view.
PDB entry 6aee coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the four Ig-like domain LILRB2 and the four-domain LILRB1 and HLA-G1 complex.
Wang Q, Song H, Cheng H, Qi J, Nam G, Tan S, Wang J, Fang M, Shi Y, Tian Z, Cao X, An Z, Yan J, Gao GF
Cell Mol Immunol 17 966-975 (2020)
PMID: 31273318

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-138260 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class I histocompatibility antigen, alpha chain G Chains: A, D
Molecule details ›
Chains: A, D
Length: 277 amino acids
Theoretical weight: 32.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17693 (Residues: 25-300; Coverage: 88%)
Gene names: HLA-6.0, HLA-G, HLAG
Sequence domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Histone H2A type 1-B/E Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04908 (Residues: 78-86; Coverage: 7%)
Gene names: H2AC4, H2AC8, H2AFA, H2AFM, HIST1H2AB, HIST1H2AE
Leukocyte immunoglobulin-like receptor subfamily B member 1 Chains: G, H
Molecule details ›
Chains: G, H
Length: 399 amino acids
Theoretical weight: 44.14 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8NHL6 (Residues: 25-417; Coverage: 63%)
Gene names: ILT2, LILRB1, LIR1, MIR7
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 69.683Å b: 154.681Å c: 98.198Å
α: 90° β: 102.24° γ: 90°
R-values:
R R work R free
0.223 0.22 0.266
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided
  • Trichoplusia ni

Quick links

Citations

9 review citations

The Molecular and Functional Characteristics of HLA-G and the Interaction with Its Receptors: Where to Intervene for Cancer Immunotherapy?
Attia et al. (2020)
8 more

1 mention without citation

BND-22, a first-in-class humanized ILT2-blocking antibody, promotes antitumor immunity and tumor regression.
Mandel et al. (2022)