› 6agj

X-ray diffraction
3Å resolution

Crystal Structure of EFHA2 in Apo State

Released:
DOI: 10.2210/pdb6agj/pdb
PDB entry 6agj coloured by chain, front view.
PDB entry 6agj coloured by chain, side view.
PDB entry 6agj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dimerization of MICU Proteins Controls Ca2+ Influx through the Mitochondrial Ca2+ Uniporter.
Xing Y, Wang M, Wang J, Nie Z, Wu G, Yang X, Shen Y
Cell Rep 26 1203-1212.e4 (2019)
PMID: 30699349

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium uptake protein 3, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 382 amino acids
Theoretical weight: 44.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86XE3 (Residues: 133-512; Coverage: 72%)
Gene names: EFHA2, MICU3
Sequence domains: EF-hand domain pair

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P41212
Unit cell:
a: 72.363Å b: 72.363Å c: 339.751Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.218 0.286
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Mitochondrial calcium exchange in physiology and disease.
Garbincius et al. (2022)
8 more

2 mentions without citation

Structures reveal gatekeeping of the mitochondrial Ca2+ uniporter by MICU1-MICU2.
Wang et al. (2020)
1 more