› 6akd

X-ray diffraction
2.1Å resolution

Crystal structure of IdnL7

Released:
DOI: 10.2210/pdb6akd/pdb
PDB entry 6akd coloured by chain, front view.
PDB entry 6akd coloured by chain, side view.
PDB entry 6akd coloured by chain, top view.
Source organism: Streptomyces sp. ML694-90F3
Primary publication:
Functional and structural characterization of IdnL7, an adenylation enzyme involved in incednine biosynthesis.
Cieślak J, Miyanaga A, Takaishi M, Kudo F, Eguchi T
Acta Crystallogr F Struct Biol Commun 75 299-306 (2019)
PMID: 30950831

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AMP-dependent synthetase/ligase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 538 amino acids
Theoretical weight: 57.51 KDa
Source organism: Streptomyces sp. ML694-90F3
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A077KUW8 (Residues: 1-522; Coverage: 100%)
Gene name: idnL7
Sequence domains: AMP-binding enzyme

Ligands and Environments

2 bound ligands:
Ligand A5A 1 x A5A
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P41212
Unit cell:
a: 68.526Å b: 68.526Å c: 225.169Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.22
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Polyene Macrolactams from Marine and Terrestrial Sources: Structure, Production Strategies, Biosynthesis and Bioactivities.
Zhao et al. (2022)

1 mention without citation

Functional and structural characterization of IdnL7, an adenylation enzyme involved in incednine biosynthesis.
Cieślak et al. (2019)