› 6ako

X-ray diffraction
2.4Å resolution

Crystal Structure of FOXC2 DBD Bound to DBE2 DNA

Released:
DOI: 10.2210/pdb6ako/pdb
PDB entry 6ako coloured by chain, front view.
PDB entry 6ako coloured by chain, side view.
PDB entry 6ako coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for DNA recognition by FOXC2.
Chen X, Wei H, Li J, Liang X, Dai S, Jiang L, Guo M, Qu L, Chen Z, Chen L, Chen Y
Nucleic Acids Res 47 3752-3764 (2019)
PMID: 30722065

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119559 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein C2 Chain: C
Molecule details ›
Chain: C
Length: 103 amino acids
Theoretical weight: 12.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99958 (Residues: 72-172; Coverage: 20%)
Gene names: FKHL14, FOXC2, MFH1
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(CP*AP*AP*AP*AP*TP*GP*TP*AP*AP*AP*CP*AP*AP*GP*A)-3') Chain: A
Molecule details ›
Chain: A
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
DNA (5'-D(TP*CP*TP*TP*GP*TP*TP*TP*AP*CP*AP*TP*TP*TP*TP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Cu FINE FOCUS
Spacegroup: P212121
Unit cell:
a: 42.54Å b: 72.924Å c: 99.047Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.25
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Metastasis and MAPK Pathways.
Kciuk et al. (2022)
2 more

4 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
3 more