› 6akv

X-ray diffraction
2.4Å resolution

Crystal structure of LysB4, the endolysin from Bacillus cereus-targeting bacteriophage B4

Released:
DOI: 10.2210/pdb6akv/pdb
PDB entry 6akv coloured by chain, front view.
PDB entry 6akv coloured by chain, side view.
PDB entry 6akv coloured by chain, top view.
Source organism: Bacillus phage B4
Primary publication:
Crystal Structure of LysB4, an Endolysin from Bacillus cereus-Targeting Bacteriophage B4.
Hong S, Son B, Ryu S, Ha NC
Mol Cells 42 79-86 (2019)
PMID: 30518175

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124964 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SH3b domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 30.08 KDa
Source organism: Bacillus phage B4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: H9NAL3 (Residues: 1-262; Coverage: 100%)
Gene names: BCB4_0006, lysB4
Sequence domains:
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: I41
Unit cell:
a: 79.457Å b: 79.457Å c: 77.925Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.176 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Structural and functional insights into a novel two-component endolysin encoded by a single gene in Enterococcus faecalis phage.
Zhou et al. (2020)
1 more

1 mention without citation

Crystal Structure of LysB4, an Endolysin from Bacillus cereus-Targeting Bacteriophage B4.
Hong et al. (2019)