› 6ale

X-ray diffraction
2.5Å resolution

A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model

Released:
DOI: 10.2210/pdb6ale/pdb
PDB entry 6ale coloured by chain, front view.
PDB entry 6ale coloured by chain, side view.
PDB entry 6ale coloured by chain, top view.
Source organisms:
Primary publication:
A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model.
Nam YW, Baskoylu SN, Gazgalis D, Orfali R, Cui M, Hart AC, Zhang M
Sci Rep 8 10749 (2018)
PMID: 30013223

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144040 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Small conductance calcium-activated potassium channel protein 2 Chain: B
Molecule details ›
Chain: B
Length: 95 amino acids
Theoretical weight: 11.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H2S1 (Residues: 394-486; Coverage: 16%)
Gene name: KCNN2
Sequence domains: Calmodulin binding domain
Structure domains: Helix Hairpins
Calmodulin-2 Chain: R
Molecule details ›
Chain: R
Length: 146 amino acids
Theoretical weight: 16.42 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP30 (Residues: 5-148; Coverage: 97%)
Gene names: CaMII, Calm2, Cam2, Camb
Sequence domains: EF-hand domain pair

Ligands and Environments

4 bound ligands:
Ligand SO4 4 x SO4
Ligand 1KP 1 x 1KP
Ligand GOL 1 x GOL
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: C2
Unit cell:
a: 76.653Å b: 66.913Å c: 64.712Å
α: 90° β: 92.47° γ: 90°
R-values:
R R work R free
0.191 0.188 0.255
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Atomistic Insights of Calmodulin Gating of Complete Ion Channels.
Núñez et al. (2020)
1 more

1 mention without citation

A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model.
Nam et al. (2018)