› 6ank

X-ray diffraction
2.25Å resolution

Synaptotagmin-7, C2A- and C2B-domains

Released:
DOI: 10.2210/pdb6ank/pdb
PDB entry 6ank coloured by chain, front view.
PDB entry 6ank coloured by chain, side view.
PDB entry 6ank coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Exceptionally tight membrane-binding may explain the key role of the synaptotagmin-7 C2A domain in asynchronous neurotransmitter release.
Voleti R, Tomchick DR, Südhof TC, Rizo J
Proc Natl Acad Sci U S A 114 E8518-E8527 (2017)
PMID: 28923929

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178963 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Synaptotagmin-7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 33 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q62747 (Residues: 134-403; Coverage: 67%)
Gene name: Syt7
Sequence domains: C2 domain

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 60.637Å b: 75.059Å c: 70.98Å
α: 90° β: 114.51° γ: 90°
R-values:
R R work R free
0.218 0.214 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Mechanism of neurotransmitter release coming into focus.
Rizo J. (2018)
8 more

5 mentions without citation

Exceptionally tight membrane-binding may explain the key role of the synaptotagmin-7 C2A domain in asynchronous neurotransmitter release.
Voleti et al. (2017)
4 more