› 6anw

X-ray diffraction
2.49Å resolution

Crystal structure of anti-CRISPR protein AcrF10

Released:
DOI: 10.2210/pdb6anw/pdb
PDB entry 6anw coloured by chain, front view.
PDB entry 6anw coloured by chain, side view.
PDB entry 6anw coloured by chain, top view.
Source organism: Shewanella xiamenensis
Primary publication:
Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex.
Guo TW, Bartesaghi A, Yang H, Falconieri V, Rao P, Merk A, Eng ET, Raczkowski AM, Fox T, Earl LA, Patel DJ, Subramaniam S
Cell 171 414-426.e12 (2017)
PMID: 28985564

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
anti-CRISPR protein AcrF10 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 99 amino acids
Theoretical weight: 11.38 KDa
Source organism: Shewanella xiamenensis
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 73.774Å b: 106.618Å c: 95.349Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Cryo-EM in drug discovery: achievements, limitations and prospects.
Renaud et al. (2018)
26 more

4 mentions without citation

Keeping crispr in check: diverse mechanisms of phage-encoded anti-crisprs.
Trasanidou et al. (2019)
3 more