› 6ap6

X-ray diffraction
1.65Å resolution

Crystal Structure of DAD2 in complex with tolfenamic acid

Released:
DOI: 10.2210/pdb6ap6/pdb
PDB entry 6ap6 coloured by chain, front view.
PDB entry 6ap6 coloured by chain, side view.
PDB entry 6ap6 coloured by chain, top view.
Source organism: Petunia x hybrida
Primary publication:
Inhibition of strigolactone receptors by N-phenylanthranilic acid derivatives: Structural and functional insights.
Hamiaux C, Drummond RSM, Luo Z, Lee HW, Sharma P, Janssen BJ, Perry NB, Denny WA, Snowden KC
J Biol Chem 293 6530-6543 (2018)
PMID: 29523686

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125406 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable strigolactone esterase DAD2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.89 KDa
Source organism: Petunia x hybrida
Expression system: Escherichia coli
UniProt:
  • Canonical: J9U5U9 (Residues: 1-267; Coverage: 100%)
Gene name: DAD2
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand TLF 2 x TLF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P1
Unit cell:
a: 36.86Å b: 55.83Å c: 69.23Å
α: 95.76° β: 95.13° γ: 108.46°
R-values:
R R work R free
0.17 0.168 0.2
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The mechanism of host-induced germination in root parasitic plants.
Nelson DC. (2021)
4 more

1 mention without citation

Inhibition of strigolactone receptors by N-phenylanthranilic acid derivatives: Structural and functional insights.
Hamiaux et al. (2018)