› 6ap7

X-ray diffraction
1.51Å resolution

Crystal Structure of DAD2 in complex with 2-(2-methyl-3-nitroanilino)benzoic acid

Released:
DOI: 10.2210/pdb6ap7/pdb
PDB entry 6ap7 coloured by chain, front view.
PDB entry 6ap7 coloured by chain, side view.
PDB entry 6ap7 coloured by chain, top view.
Source organism: Petunia x hybrida
Primary publication:
Inhibition of strigolactone receptors by N-phenylanthranilic acid derivatives: Structural and functional insights.
Hamiaux C, Drummond RSM, Luo Z, Lee HW, Sharma P, Janssen BJ, Perry NB, Denny WA, Snowden KC
J Biol Chem 293 6530-6543 (2018)
PMID: 29523686

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125406 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable strigolactone esterase DAD2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.89 KDa
Source organism: Petunia x hybrida
Expression system: Escherichia coli
UniProt:
  • Canonical: J9U5U9 (Residues: 1-267; Coverage: 100%)
Gene name: DAD2
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand BNY 2 x BNY
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P1
Unit cell:
a: 36.697Å b: 48.315Å c: 71.942Å
α: 82.67° β: 86.76° γ: 69.95°
R-values:
R R work R free
0.166 0.164 0.197
Expression system: Escherichia coli

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Citations

5 review citations

The mechanism of host-induced germination in root parasitic plants.
Nelson DC. (2021)
4 more

1 mention without citation

Inhibition of strigolactone receptors by N-phenylanthranilic acid derivatives: Structural and functional insights.
Hamiaux et al. (2018)