› 6ap8

X-ray diffraction
1.27Å resolution

Crystal Structure of rice D14 bound to 2-(2-methyl-3-nitroanilino)benzoic acid

Released:
DOI: 10.2210/pdb6ap8/pdb
PDB entry 6ap8 coloured by chain, front view.
PDB entry 6ap8 coloured by chain, side view.
PDB entry 6ap8 coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Inhibition of strigolactone receptors by N-phenylanthranilic acid derivatives: Structural and functional insights.
Hamiaux C, Drummond RSM, Luo Z, Lee HW, Sharma P, Janssen BJ, Perry NB, Denny WA, Snowden KC
J Biol Chem 293 6530-6543 (2018)
PMID: 29523686

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strigolactone esterase D14 Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.37 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q10QA5 (Residues: 52-318; Coverage: 84%)
Gene names: D14, D88, HTD2, LOC_Os03g10620, Os03g0203200
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand BNY 2 x BNY
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 48.005Å b: 88.433Å c: 119.026Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.121 0.119 0.154
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The mechanism of host-induced germination in root parasitic plants.
Nelson DC. (2021)
4 more

2 mentions without citation

Inhibition of strigolactone receptors by N-phenylanthranilic acid derivatives: Structural and functional insights.
Hamiaux et al. (2018)
1 more