› 6asb

X-ray diffraction
2.85Å resolution

CXXC and PHD-type zinc finger regions of FBXL19 in complex with DNA

Released:
DOI: 10.2210/pdb6asb/pdb
PDB entry 6asb coloured by chain, front view.
PDB entry 6asb coloured by chain, side view.
PDB entry 6asb coloured by chain, top view.
Source organisms:
Primary publication:
DNA Sequence Recognition of Human CXXC Domains and Their Structural Determinants.
Xu C, Liu K, Lei M, Yang A, Li Y, Hughes TR, Min J
Structure 26 85-95.e3 (2018)
PMID: 29276034

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119581 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
F-box/LRR-repeat protein 19 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 123 amino acids
Theoretical weight: 13.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PCT2 (Residues: 31-153; Coverage: 18%)
Gene names: FBL19, FBXL19
Sequence domains:
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
DNA (5'-D(*GP*CP*CP*AP*AP*CP*GP*TP*TP*GP*GP*C)-3') Chains: A, B, D, E, G, H, J, K
Molecule details ›
Chains: A, B, D, E, G, H, J, K
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 16 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 55.729Å b: 64.282Å c: 67.324Å
α: 103.53° β: 104.66° γ: 99.4°
R-values:
R R work R free
0.215 0.213 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Understanding the interplay between CpG island-associated gene promoters and H3K4 methylation.
Hughes et al. (2020)
8 more

2 mentions without citation

Detecting and interpreting DNA methylation marks.
Ren et al. (2018)
1 more