› 6asv

X-ray diffraction
2.21Å resolution

E. coli PRPP Synthetase

Released:
DOI: 10.2210/pdb6asv/pdb
PDB entry 6asv coloured by chain, front view.
PDB entry 6asv coloured by chain, side view.
PDB entry 6asv coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Crystal structure of E. coli PRPP synthetase.
Zhou W, Tsai A, Dattmore DA, Stives DP, Chitrakar I, D'alessandro AM, Patil S, Hicks KA, French JB
BMC Struct Biol 19 1 (2019)
PMID: 30646888

Function and Biology Details

Reaction catalysed: 
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141507 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-phosphate pyrophosphokinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 335 amino acids
Theoretical weight: 36.43 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A717 (Residues: 1-315; Coverage: 100%)
Gene names: JW1198, b1207, prs, prsA
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand PO4 6 x PO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 104.757Å b: 138.436Å c: 137.438Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.212
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Deciphering the Metabolic Pathway Difference Between Saccharopolyspora pogona and Saccharopolyspora spinosa by Comparative Proteomics and Metabonomics.
Rang et al. (2020)
5 more

2 mentions without citation

Crystal structure of E. coli PRPP synthetase.
Zhou et al. (2019)
1 more