› 6at7

X-ray diffraction
2.49Å resolution

Phenylalanine Ammonia-Lyase (PAL) from Sorghum bicolor

Released:
DOI: 10.2210/pdb6at7/pdb
PDB entry 6at7 coloured by chain, front view.
PDB entry 6at7 coloured by chain, side view.
PDB entry 6at7 coloured by chain, top view.
Source organism: Sorghum bicolor
Primary publication:
Biochemical and Structural Analysis of Substrate Specificity of a Phenylalanine Ammonia-Lyase.
Jun SY, Sattler SA, Cortez GS, Vermerris W, Sattler SE, Kang C
Plant Physiol 176 1452-1468 (2018)
PMID: 29196539

Function and Biology Details

Reaction catalysed: 
L-phenylalanine = trans-cinnamate + ammonia
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-111539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine ammonia-lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 702 amino acids
Theoretical weight: 75.66 KDa
Source organism: Sorghum bicolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5XXT8 (Residues: 1-704; Coverage: 100%)
Gene name: SORBI_3004G220300
Sequence domains: Aromatic amino acid lyase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NH4 2 x NH4
1 modified residue:
Ligand MDO 2 x MDO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: I4122
Unit cell:
a: 126.304Å b: 126.304Å c: 337.477Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.202
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Trichoderma: The Current Status of Its Application in Agriculture for the Biocontrol of Fungal Phytopathogens and Stimulation of Plant Growth.
Tyśkiewicz et al. (2022)
8 more