› 6avn

X-ray diffraction
2.5Å resolution

Crystal structure of unbound anti-HIV antibody Fab PGV19 at 2.5 A

Released:
DOI: 10.2210/pdb6avn/pdb
PDB entry 6avn coloured by chain, front view.
PDB entry 6avn coloured by chain, side view.
PDB entry 6avn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.
Sarkar A, Bale S, Behrens AJ, Kumar S, Sharma SK, de Val N, Pallesen J, Irimia A, Diwanji DC, Stanfield RL, Ward AB, Crispin M, Wyatt RT, Wilson IA
Nat Commun 9 1956 (2018)
PMID: 29769533

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231475 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PGV19 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 223 amino acids
Theoretical weight: 24.25 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
PGV19 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 209 amino acids
Theoretical weight: 22.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 69.783Å b: 69.783Å c: 193.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.25
Expression system: Homo sapiens

Quick links

Citations

11 review citations

The Proteolytic Regulation of Virus Cell Entry by Furin and Other Proprotein Convertases.
Izaguirre G. (2019)
10 more

1 mention without citation

Structure of a cleavage-independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer.
Sarkar et al. (2018)